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動物生殖学研究室

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研究課題

1. 家畜における卵子発生能獲得に関する研究と高品質胚の体外胚生産系の確立 2. 卵子および胚の低温(ガラス化)保存法の開発 3. 発育途上卵子の体外発育培養技術の開発 4. 哺乳動物精子の凍結保存と受精能獲得に関する研究 5. 人工繁殖技術の野生動物への応用

トピックス

2018年 新規ガラス化保存デバイスの製品化の実現(三菱製紙株式会社との共同研究) 2019年 仙台市立八木山動物公園と「動物園で飼育される動物への人工繁殖技術の応用に関する研究」について共同研究協定締結 2020年 JRA畜産振興事業「牛ガラス化胚の新規移植法開発・実用化事業」採択(令和2~4年度)

研究業績

Furukawa, E., Masaki, T., Sakaguchi, K., Bo, M., Yanagawa, Y., Ueda, K. and Nagano, M: Relationship between the timing of the first postpartum ovulation and antral follicle counts in Holstein cows, Journal of Ovarian Research, 13: 7, 2020.

Chen, Z., Wu, Y., Nagano, M. †, Ueshiba, K., Furukawa, E., Yamamoto, Y., Chiba, H. and Hui, S.P.: Lipidomic profiling of dairy cattle oocytes by high performance liquid chromatography-high resolution tandem mass spectrometry for developmental competence markers, Theriogenology, 144: 56-66, 2020.

Yang, Y., Kanno, C., Sakaguchi, K., Katagiri, S., Yanagawa, Y. and Nagano, M. *: Theca cells can support bovine oocyte growth in vitro without the addition of steroid hormones, Theriogenology, 142; 41-47, 2020.

Takahashi, N., Harada, M., Ohi, N., Izumi, G., Momozawa, K., Matsuzawa, A., Tokunaga, Y., Hirata, T., Fujii, T. and Osuga, Y. 2020. Preclinical validation of the new vitrification device possessing a feature of absorbing excess vitrification solution for the cryopreservation of human embryos. J. Obstet. Gynaecol. Res. , 46: 302-309.

Sakaguchi, K., Yanagawa, Y., Yoshioka, K., Suda, T., Katagiri, S. and Nagano, M. *: Relationships between the antral follicle count, steroidogenesis, and secretion of follicle-stimulating hormone and anti-M?llerian hormone during follicular growth in cattle, Reproductive Biology and Endocrinology, 17: 88, 2019.

Abe, S., Koyanagi, Y., Nakano, M., Sasaki, M., Matsuzawa, A., Tokunaga, Y., Yoshida, K., *Momozawa, K. and Miyake, T. 2019. Usefulness of vitrification device having vitrification solution absorber for cryopreservation of mouse embryos at the blastocyst stage. Jpn. J. Vet. Res. 68: 59-64.

Torii, Y., Matsumoto, N., Sakamoto, H., Nagano, M., Katagiri, S. and Yanagawa, Y.: Monitoring follicular dynamics using ultrasonography in captive brown bears (Ursus arctos) during the breeding season, Theriogenology, 140: 164-170, 2019.

Nagano M. Acquisition of developmental competence and in vitro growth culture of bovine oocytes. J Reprod Dev 65: 195-201, 2019.

Momozawa K, Matsuzawa A, Tokunaga Y, Ohi N, Harada M. A new vitrification device that absorbs excess vitrification solution adaptable to a closed system for the cryopreservation of mouse embryos. Cryobiology 88: 9-14, 2019.

Sakaguchi K, Maylem ERS, Tilwani RC, Yanagawa Y, Katagiri S, Atabay EC, Atabay EP, Nagano M. Effects of follicle-stimulating hormone followed by gonadotropin-releasing hormone on embryo production by ovum pick-up and in vitro fertilization in the river buffalo (Bubalus bubalis). Anim Sci J 90: 690-695, 2019.

Abdel-Ghani MA, Yanagawa Y, Balboula AZ, Sakaguchi K, Kanno C, Katagiri S, Takahashi M, Nagano M. Astaxanthin improves the developmental competence of in vitro grown oocytes and modifies the steroidogenesis of granulosa cells derived from bovine early antral follicles. Reprod Fertil Dev 31: 272-281, 2019.

Tomiyasu J, Yanagawa Y, Sato Y, Shimozuru M, Nagano M, Sasaki M, Sakamoto H, Matsumoto N, Kobayashi K, Kayano M, Haneda S, Matsui M. Testosterone-related and seasonal changes in sebaceous glands in the back skin of adult male brown bears (Ursus arctos). Can J Zool 96: 205-211, 2018.

Kanno C, Sakamoto KQ, Yanagawa Y, Takahashi Y, Katagiri S, Nagano M. Comparison of sperm subpopulation structures in first and second ejaculated semen from Japanese black bulls by a cluster analysis of sperm motility evaluated by a CASA system. J Vet Med Sci 79: 1359-1365, 2017.

Sakaguchi K, Huang W, Yang Y, Yanagawa Y, Nagano M. Relationship between in vitro growth of bovine oocytes and steroidogenesis of granulosa cells cultured in medium supplemented with bone morphogenetic protein-4 and follicle stimulating hormone. Theriogenology 97: 113-123, 2017.

Momozawa K, Matuzawa A, Tokunaga Y, Abe S, Koyanagi Y, Kurita M, Nakano M, Miyake T. Efficient vitrification of mouse embryos using the Kitasato Vitrification System as a novel vitrification device. Reprod Biol Endocrinol 15: 29, 2017.

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