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1. 乳酸菌やビフィズス菌の腸内定着機構の解析 2. 乳酸菌やビフィズス菌の抗酸化機能の解析と分子育種 3. コアラなど希少動物の腸内菌叢の解析 4. 食による腸内細菌叢を介した宿主の生体恒常性維持機構の評価発 5. リステリアなどの食中毒菌の病原因子の解析
[学術論文]
1) Kashimoto T, Sugiyama H, Kawamidori K, Yamazaki K, Kado T, Matsuda K, Kodama T, Mukai T, Ueno S. Vibiro vulnificus hemolysin associates with gangliosides. BMC Microbiol. 20(1):69. doi: 10.1186/s12866-020-01755-1.(2020)
2) Nishiyama K, Mukai T. Adhesion of Lactobacillus to Intestinal Mucin. Methods Mol Biol. 1887:159-166. doi: 10.1007/978-1-4939-8907-2_14. (2019)
3) Nishiyama K, Sugiyama M, Yamada H, Makino K, Ishihara S, Takaki T, Mukai T, Okada N. A new approach for analyzing an adhesive bacterial protein in the mouse gastrointestinal tract using optical tissue clearing., Scientific Reports 9. 4731, doi: 10.1038/s41598-019-41151-y (2019)
4) Nishiyama K, Nagai A, Uribayashi K, Yamamoto Y, Mukai T, Okada N. Two extracellular sialidases from Bifidobacterium bifidum promote the degradation of sialyl-oligosaccharides and support the growth of Bifidobacterium breve. Anaerobe, 52, 22-28 (2018)
5) 西山啓太,向井孝夫. ビフィズス菌と宿主腸粘膜との相互作用に関わる因子. 日本乳酸菌学会誌 29, 13-18 (2018)
6) Nishiyama K, Yamamoto Y, Sugiyama M, Takaki T, Urashima T, Fukiya S, Yokota A, Okada N, Mukai T, Bifidobacterium bifidum extracellular sialidase enhances adhesion to the mucosal surface and supports carbohydrate assimilation. mBio, 8/ 5, e00928-17,(2017)
7) Nishiyama K, Kagamitani T, Yamamoto Y, Okada N, Mukai T, The elongation factor Tu from Lactobacillus reuteri inhibits the adhesion of Helicobacter pylori to porcine gastric mucin. Milk Science,.66,1 (2017)
8) Aryantini NP, Kondoh D, Nishiyama, K, Yamamoto Y, Mukai T, Sujaya IN, Urashima T, Fukuda K, Anchorless cell surface proteins function as laminin-binding adhesins in Lactobacillus rhamnosus FSMM22. FEMS Microbiol. Lett., 364,4, doi:
9) Nishiyama K, Sugiyama M, Mukai T. Adhesion Properties of Lactic Acid Bacteria on Intestinal Mucin. Microorganisms, 4/ 3, 34 (2016)
10) Nishiyama K, Sugiyama M, Mukai T. Adhesion Properties of Lactic Acid Bacteria on Intestinal Mucin. Microorganisms, 4/ 3, 34 (2016)
11) 向井孝夫,西山啓太.:プロバイオティクスにおける最近の研究動向 ~乳酸菌やビフィズス菌のムチンとの相互作用~.乳業技術.65, 23-40, (2016)
12) 西山啓太,向井孝夫.乳酸菌の腸粘膜への定着機構.化学と生物,54,7,471-477 (2016)
13) Nishiyama, K., Ueno, S., Sugiyama, M., Yamamoto, Y., and Mukai, T.: Lactobacillus rhamnosus GG SpaC pilin subunit binds to the carbohydrate moieties of intestinal glycoconjugates. Anim. Sci J. 87,6,809-815, doi: 10.1111/asj.12491 (2016)
14) 西山啓太,向井孝夫.乳酸菌によるカンピロバクターの感染予防とその作用機序解明に向けた研究.畜産技術,730, 2-6 (2016)
15) Suzuki, K., Nishiyama, K., Miyajima, H., Osawa, R., Yamamoto, Y., and Mukai, T.: Adhesion properties of a putative polymorphic fimbrial subunit protein from Bifidobacterium longum subsp. longum. Biosci, Microbiota Food Health, 35,19-27(2016)
16) 西山啓太,向井孝夫.プロバイオティクスによる病原細菌の感染予防.ミルクサイエンス, 64, 3, 245-253 (2015)
17) Nishiyama, K., Nakazato, A., Ueno, S., Seto, Y., Kakuda, T., Takai, S., Yamamoto, Y., and Mukai, T.: Cell surface-associated aggregation-promoting factor from Lactobacillus gasseri SBT2055 facilitates host colonization and competitive exclusion of Campylobacter jejuni. Mol. Microbiol., 98,712-726(2015)
18) Nishiyama K, Nakamata K, Ueno S, Terao A, Aryantini NP, Sujaya IN, Fukuda K, Urashima T, Yamamoto Y, and Mukai T. Adhesion properties of Lactobacillus rhamnosus mucus-binding factor to mucin and extracellular matrix proteins. Biosci Biotechnol Biochem.,78,271-279(2015)
19) Matsui, H., Takahashi, T., Øverby, A., Murayama, S. Y., Yoshida, H., Yamamoto, Y., Nishiyama, K., Seto, Y., Takahashi, T., Mukai, T., and Nakamura, M.:Mouse models for assessing the protective efficacy of Lactobacillus gasseri SBT2055 against Helicobacter suis infection associated with the development of gastric mucosa-associated lymphoid tissue lymphoma. Helicobacter, 20, 291-298(2015)
20) 西山啓太,向井孝夫:乳酸菌とムチンの相互作用 -糖鎖を介した乳酸菌の腸粘膜への付着機構-.応用糖質科学.5.61-65(2015)
21) Nishiyama, K., Seto, Y., Yoshioka, K., Kakuda, T., Takai, S., Yamamoto, Y., and Mukai, T.: Lactobacillus gasseri SBT2055 reduces infection by and colonization of Campylobacter jejuni. PloS ONE, 29, e108827(2014)
22) Sasaki, Y., Horiuchi, H., Kawashima, H., Mukai, T., and Yamamoto, Y.: NADH Oxidase of Streptococcus thermophilus 1131 is Required for the effective yogurt fermentation with Lactobacillus delbrueckii subsp. bulgaricus 2038. Biosci. Microbiota Food Health, 33,31-40(2014)
23) Nishiyama, K., Kawanabe, A., Miyauchi, H., Abe, F., Tsubokawa, D., Ishihara, K., Yamamoto, Y., and Mukai, T.: Evaluation of bifidobacterial adhesion to acidic sugar chains of porcine colonic mucins. Biosci. Biotechnol. Biochem.,78,1444–1451 (2014)
24) Nishiyama, K., Ochiai, A., Tsubokawa, D., Ishihara, K., Yamamoto, Y., and Mukai, T.: Identification and characterization of sulfated carbohydrate-binding protein from Lactobacillus reuteri. PLoS One, 8, e83703 (2013)
25) 山本裕司,向井孝夫.: ヨーグルト乳酸菌の生態学と人類への恩恵.ビオストーリー,19,34-40(2013)
26) Shi, T., Nishiyama, K., Nakamata, K., Aryantini, N.P., Mikumo, D., Oda, Y., Yamamoto, Y., Mukai, T., Sujaya, I.N., Urashima, T., and Fukuda, K.: Isolation of potential probiotic Lactobacillus rhamnosus strains from traditional fermented mare milk produced in Sumbawa Island of Indonesia. Biosci Biotechnol Biochem. 76(10):pp.1897—1903. (2012).
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